ALEXA FLUOR 555 Rabbit anti- ACA11 Polyclonal Antibody, ALEXA FLUOR 555 Conjugated [IgG]

calcium-transporting ATPase; Putative calcium-transporting ATPase 11, plasma membrane-type; Ca2+-ATPase isoform 11; autoinhibited Ca2+-ATPase 11Arabidopsis thaliana; ACA11_ARATH.

In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants.